Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils cultivated large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine degree relationship between 23 landraces one registered variety. Methods: To take samples from populations, seeds were sown field 2014. Samples taken young leaves plants preserved at -80oC same year. RAPD ISSR primers used study. The bands obtained as result PCR recorded data both methods also evaluated by combining them. Result: In study, 5 4 total 49 obtained. Of 29 using primers, 20 found be polymorphic 15 polymorphic. It there very low correlation two methods. Using markers + combination, similarity index among populations 0.25-0.95, 0.5-1.00 0.45-0.91, respectively. Nei’s 0.3365, 0.2656 0.3018 with RAPD, Based dendrograms under analysis classified into 3, 3 groups, With this closest identified significantly detected.